Goldstein L S, Spindle A I
Mutat Res. 1976 Dec;41(2-3):289-96. doi: 10.1016/0027-5107(76)90102-0.
The expression of dominant lethal mutations induced by 450 rad of X-radiation in the germ cells of male mice was detected by an in vitro analysis. Embryos sired by irradiated males had a higher incidence of developmental failures in early cleavage, at the late morula stage, and at the late blastocyst stage due to dominant lethal mutations than did control embryos. Dominant lethal mutations were induced most frequently in germ cells exposed as early spermatids and spermatocytes and next most frequently in germ cells exposed as sperm. A decrease in fertilizations, presumably reflecting cell killing, was found in irradiated spermatocytes. These observations indicate that in vitro analysis permits a more detailed description of mutations that are expressed as preimplantation failures than does in vivo analysis.
通过体外分析检测了450拉德X射线辐射诱导雄性小鼠生殖细胞中显性致死突变的表达。受辐射雄性小鼠所生的胚胎，由于显性致死突变，在早期卵裂期、桑葚胚晚期和囊胚晚期出现发育失败的发生率高于对照胚胎。显性致死突变最常发生在作为早期精子细胞和精母细胞暴露的生殖细胞中，其次最常发生在作为精子暴露的生殖细胞中。在受辐射的精母细胞中发现受精率下降，这可能反映了细胞杀伤。这些观察结果表明，与体内分析相比，体外分析能够更详细地描述表现为植入前失败的突变。
