Muszyński Z, Długaszewska J
Katedra i Zakład Bakteriologii Farmaceutycznej AM w Poznaniu.
Med Dosw Mikrobiol. 1994;46(1-2):87-90.
Investigations were carried on with model cream with O/W and W/O emulsion type and three selected tooth pastes produced by Pollena-Lechia. Own "conservation test" was applied for a period of 28 days basing on methods recommended by PN-80/C-77022 for cosmetics. Applied test microorganisms were recommended by National Institute of Hygiene for investigation of disinfecting agents and strains of bacteria and fungi isolated from cosmetics. Initial level of contamination amounted to 10(6) cells/g. Following biocides were used: Nipagin A, M and P, Kathon CG, Sepicide Cl, Chlor-hexidine gluconate, Myacide SP, Bromochlorophene, Germall 115, Gropol, benzalkonium bromide, Cetrimide, Irgasan DP-300 and Bronopol. Most effective biocides for creams with W/O emulsion type effective during 5 days were Gropol and Bronopol in concentration of 0.01% and Kathon CG in concentration of 0.05%. For tooth pastes optimal biocide was Sepicide Cl in concentration of 0.3% which was cidal in time of 4 days. Out of tested microorganisms most resistant to mentioned biocides were Pseudomonas aeruginosa i P. putida isolated from cosmetic creams.
对具有O/W和W/O乳液类型的模型乳膏以及由Pollena-Lechia生产的三种选定牙膏进行了研究。根据PN-80/C-77022推荐的化妆品方法，进行了为期28天的自行“保存测试”。应用的测试微生物由国家卫生研究所推荐，用于消毒剂调查以及从化妆品中分离出的细菌和真菌菌株。初始污染水平为10(6)个细胞/克。使用了以下防腐剂：尼泊金A、M和P、凯松CG、Sepicide Cl、葡萄糖酸洗必泰、Myacide SP、溴氯酚、Germall 115、Gropol、苯扎溴铵、西曲溴铵、Irgasan DP-300和溴硝醇。对于W/O乳液类型的乳膏，在5天内最有效的防腐剂是浓度为0.01%的Gropol和溴硝醇以及浓度为0.05%的凯松CG。对于牙膏，最佳防腐剂是浓度为0.3%的Sepicide Cl，其在4天内具有杀菌作用。在测试的微生物中，对上述防腐剂最具抗性的是从化妆品乳膏中分离出的铜绿假单胞菌和恶臭假单胞菌。
