逐步氧化14C-棕榈酸并释放14CO2所需的人源麻风分枝杆菌数量的滴定。
Titration of numbers of human-derived Mycobacterium leprae required to progressively oxidize 14C-palmitic acid and release 14CO2.
作者信息
Shannon E J, Frommel D, Guebre-Xabier M, Haile-Mariam H S
机构信息
Gillis W. Long Hansen's Disease Center, Carville, Louisiana.
出版信息
Lepr Rev. 1994 Jun;65(2):100-5.
Mycobacterium leprae was isolated from skin-punch biopsies of 2 untreated lepromatous leprosy patients. The bacteria were enumerated, diluted 10-fold and cultured in Middlebrook 7H9 medium supplemented with albumin, dextrose, catalase and 14C-palmitic acid. The cultures were incubated at 33 degrees C in a modified Buddemeyer radiorespiratory detection vessel. Those cultures containing at least 10(7) mycobacteria demonstrated a progressive evolution of 14CO2.
从2例未经治疗的瘤型麻风患者的皮肤打孔活检标本中分离出麻风分枝杆菌。对细菌进行计数，稀释10倍后在补充有白蛋白、葡萄糖、过氧化氢酶和14C-棕榈酸的Middlebrook 7H9培养基中培养。培养物在改良的Buddemeyer放射性呼吸检测容器中于33℃孵育。那些含有至少10(7) 分枝杆菌的培养物显示出14CO2的逐步产生。
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