Inoue O J, Lee J H, Isaacson R E
Department of Veterinary Pathobiology, University of Illinois, Urbana 61801.
Mol Microbiol. 1993 Nov;10(3):607-13. doi: 10.1111/j.1365-2958.1993.tb00932.x.
The production of the Escherichia coli pilus adhesin K99 requires the expression of eight unique proteins: FanA-H. The transcriptional organization of the K99 operon was investigated by Northern blot analysis. Four RNAs of 0.54, 1.4, 2.5 and 3.5 kb were detected. When a fanC probe was used all four RNAs were detected while the use of fanD, fanF and fanG probes detected two RNAs each. Using several deletion and TnphoA insertion mutants it was concluded that there were seven unique K99-specific transcripts, several of which were of the same approximate sizes (1.4 and 2.5 kb). It also was concluded that K99 was comprised of at least three complementation groups, two of which were regulated by catabolite repression.
大肠杆菌菌毛黏附素K99的产生需要八种独特蛋白质（FanA - H）的表达。通过Northern印迹分析研究了K99操纵子的转录组织。检测到了0.54、1.4、2.5和3.5 kb的四种RNA。当使用fanC探针时，所有四种RNA都能被检测到，而使用fanD、fanF和fanG探针时，每种只能检测到两种RNA。利用几个缺失突变体和TnphoA插入突变体得出结论，存在七种独特的K99特异性转录本，其中几种大小大致相同（1.4和2.5 kb）。还得出结论，K99至少由三个互补组组成，其中两个受分解代谢物阻遏调控。
