Barbu V, Alpha-Bazin B, Pepin D, Morand-Joubert L, Chypre C, Bereziat G
Hopital Saint-Antoine, Service de Biochimie B, Paris, France.
Mol Cell Probes. 1994 Jun;8(3):251-6. doi: 10.1006/mcpr.1994.1036.
We describe an 'immunoassay-like' detection format, based on the AMPLICIS technique for the routine detection of BCR/ABL transcripts in leukaemic patients. The AMPLICIS technique is characterized as a nested PCR coupled to solid phase isotopic or non-isotopic detection of PCR products. Comparison between this assay and a conventional PCR technique using Southern-blot analysis provided a good correlation between the two procedures. The assay, easier and faster than the conventional one, gives access to automation and therefore appears well suited for the routine screening of BCR/ABL chimaeric mRNAs.
我们描述了一种基于AMPLICIS技术的“免疫分析样”检测形式，用于白血病患者BCR/ABL转录本的常规检测。AMPLICIS技术的特点是一种嵌套式PCR技术，与PCR产物的固相同位素或非同位素检测相结合。该检测方法与使用Southern印迹分析的传统PCR技术之间的比较表明，这两种方法具有良好的相关性。该检测方法比传统方法更简便、快速，可实现自动化，因此似乎非常适合BCR/ABL嵌合mRNA的常规筛查。
