一个编码克氏锥虫中异质性组蛋白H1蛋白的基因家族。

A gene family encoding heterogeneous histone H1 proteins in Trypanosoma cruzi.

作者信息

Aslund L, Carlsson L, Henriksson J, Rydåker M, Toro G C, Galanti N, Pettersson U

机构信息

Department of Medical Genetics, Biomedical Center, Uppsala, Sweden.

出版信息

Mol Biochem Parasitol. 1994 Jun;65(2):317-30. doi: 10.1016/0166-6851(94)90082-5.

DOI:10.1016/0166-6851(94)90082-5

PMID:7969272

Abstract

A gene family encoding a set of histone H1 proteins in Trypanosoma cruzi is described. The sequence of 3 genomic and 4 cDNA clones revealed the presence of several motifs characteristic of histone H1, although heterogeneity at the polypeptide level was evident. The clones encode histone H1 proteins of an unusually small size (74-97 amino acids), which lack the globular domain found in histone H1 of higher eukaryotes. All histone H1 mRNAs from T. cruzi are polyadenylated, although no typical polyadenylation signal was found. Furthermore, the genes encoding the histone H1 proteins in T. cruzi are found in a tandem array containing 15-20 gene copies per haploid genome. This tandem array is located on a large chromosome of 2.2 Mb.

摘要

描述了一个编码克氏锥虫一组组蛋白H1蛋白的基因家族。3个基因组克隆和4个cDNA克隆的序列显示出组蛋白H1的几个特征基序的存在，尽管在多肽水平上存在明显的异质性。这些克隆编码的组蛋白H1蛋白大小异常小（74 - 97个氨基酸），缺少高等真核生物组蛋白H1中发现的球状结构域。来自克氏锥虫的所有组蛋白H1 mRNA均被多聚腺苷酸化，尽管未发现典型的多聚腺苷酸化信号。此外，克氏锥虫中编码组蛋白H1蛋白的基因以串联阵列形式存在，每个单倍体基因组包含15 - 20个基因拷贝。这个串联阵列位于一条2.2 Mb的大染色体上。