使用反义寡脱氧核苷酸进行基因抑制。
Gene inhibition using antisense oligodeoxynucleotides.
作者信息
Wagner R W
机构信息
Gilead Sciences, Foster City, California 94404.
出版信息
Nature. 1994 Nov 24;372(6504):333-5. doi: 10.1038/372333a0.
DOI:10.1038/372333a0
PMID:7969490
Abstract
Antisense oligodeoxynucleotides (ODNs) have great promise as agents for the specific manipulation of gene expression. Until recently, nonspecific effects of ODNs often confounded the interpretation of antisense studies. Improvements in ODN chemistry and cellular delivery techniques now allow for more potent and specific gene inhibition. This review critically evaluates recent progress in the development of antisense ODNs.
摘要
反义寡脱氧核苷酸（ODNs）作为特异性调控基因表达的试剂具有巨大潜力。直到最近，ODNs的非特异性效应常常使反义研究的解释变得复杂。ODN化学和细胞递送技术的改进现在使得更有效和特异的基因抑制成为可能。这篇综述批判性地评估了反义ODNs开发的最新进展。
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