Yu Bo, Meng Fanbo, Yang Yushuang, Liu Dongna, Shi Kaiyao
Department of cardiology, China-Japan union hospital of Jilin University, Changchun, Jilin, 130033, P.R. China.
Int J Med Sci. 2016 Jul 27;13(8):646-52. doi: 10.7150/ijms.15177. eCollection 2016.
Heart ischemia is a hypoxia related disease. NOX2 and HIF-1α proteins were increased in cardiomyocytes after acute myocardial infarction. However, the relationship of the hypoxia-induced HIF-1α. NOX2-derived oxidative stress and apoptosis in cardiomyocyte remains unclear. In the current study, we use NOX2 antisense strategy to investigate the role of NOX2 in hypoxia-induced oxidative stress and apoptosis in rat cardiomyocytes. Here, we show that transduction of ADV-NOX2-AS induces potent silencing of NOX2 in cardiomyocytes, and resulting in attenuation of hypoxia-induced oxidative stress and apoptosis. This study indicates the potential of antisense-based therapies and validates NOX2 as a potent therapeutic candidate for heart ischemia.
心脏缺血是一种与缺氧相关的疾病。急性心肌梗死后，心肌细胞中的NOX2和HIF-1α蛋白增加。然而，缺氧诱导的HIF-1α、NOX2衍生的氧化应激与心肌细胞凋亡之间的关系仍不清楚。在本研究中，我们采用NOX2反义策略来研究NOX2在大鼠心肌细胞缺氧诱导的氧化应激和凋亡中的作用。在此，我们表明，转导ADV-NOX2-AS可有效沉默心肌细胞中的NOX2，并减轻缺氧诱导的氧化应激和凋亡。本研究表明了基于反义的治疗方法的潜力，并验证了NOX2作为心脏缺血有效治疗候选物的可能性。
