Mosier D R, Zengel J E
Department of Veterans Affairs Medical Center, Gainesville, FL.
Neurosci Lett. 1994 Jun 6;174(1):1-4. doi: 10.1016/0304-3940(94)90104-x.
We have examined the effect of ouabain, a Na,K-ATPase inhibitor known to secondarily increase intracellular Ca2+ levels, on evoked (phasic) transmitter release at the frog neuromuscular junction. As had been reported previously, we observed an increase in both spontaneous and evoked release with prolonged exposure to ouabain. We also found that following ouabain treatment, evoked release was maintained for a much longer period of time upon removal of extracellular Ca2+ than prior to ouabain exposure. These results indicate that after exposure to ouabain evoked transmitter release can occur in the absence of appreciable Ca2+ entry.
我们研究了哇巴因（一种已知会继发增加细胞内钙离子水平的钠钾ATP酶抑制剂）对青蛙神经肌肉接头处诱发（阶段性）递质释放的影响。正如之前所报道的，我们观察到长时间暴露于哇巴因后，自发释放和诱发释放均增加。我们还发现，在哇巴因处理后，去除细胞外钙离子时，诱发释放维持的时间比哇巴因暴露前长得多。这些结果表明，暴露于哇巴因后，在没有明显钙离子内流的情况下也可发生诱发递质释放。
