Zettl U K, Gold R, Hartung H P, Toyka K V
Department of Neurology, Julius-Maximilians-Universität, Würzburg, Germany.
Neurosci Lett. 1994 Jul 18;176(1):75-9. doi: 10.1016/0304-3940(94)90875-3.
The present study describes apoptosis of T-lymphocytes in the sciatic nerve in experimental autoimmune neuritis (EAN), a T-cell mediated disorder of the peripheral nervous system (PNS). Morphological signs characteristic for apoptotic cell death were found from day 4 onwards, peaking at day 7. Apoptosis of T-cells could be detected throughout the subsequent recovery period. In situ nick translation (ISNT) followed by immunocytochemical analysis confirmed the presence of DNA fragmentation in T-lymphocytes. We thus show for the first time that apoptosis is not a unique elimination mechanism of inflammatory T-cells in the central nervous system (CNS), but is operative in the PNS as well although at a lesser degree. Identifying the underlying mechanisms may provide a basis for the development of new therapeutic strategies in autoimmune disorders.
本研究描述了实验性自身免疫性神经炎（EAN）中坐骨神经T淋巴细胞的凋亡情况，EAN是一种T细胞介导的周围神经系统（PNS）疾病。从第4天起发现了凋亡细胞死亡的形态学特征，在第7天达到峰值。在随后的整个恢复期都能检测到T细胞凋亡。原位缺口平移（ISNT）后进行免疫细胞化学分析证实了T淋巴细胞中存在DNA片段化。因此，我们首次表明凋亡并非中枢神经系统（CNS）中炎性T细胞唯一的清除机制，而是在PNS中也起作用，尽管程度较轻。确定其潜在机制可能为自身免疫性疾病新治疗策略的开发提供基础。
