Odano I, Ohkubo M, Takahashi N, Higuchi T
Department of Radiology, Niigata University School of Medicine, Japan.
Nucl Med Commun. 1994 Jul;15(7):560-4. doi: 10.1097/00006231-199407000-00011.
We developed a new method for quantitative measurement of regional cerebral blood flow (rCBF) using one-point arterial sampling with N-isopropyl-p-[123I]-iodoamphetamine (123I-IMP) and single photon emission computed tomography (SPECT) based on the microsphere model. Although the conventional microsphere method requires both the continuous withdrawal of arterial blood (integral of Ca(t)) and treatment of the blood with octanol to obtain the fraction of true tracer activity in the integral of Ca(t) (N), the new method does not require these two procedures. We examined 14 patients to analyse the correlation between the integral of Ca(t)N and a small arterial sample obtained at one time point [one-point Ca(t)] after the injection of 123I-IMP without octanol treatment. The integral of Ca(t)N was calculated from one point Ca(t) using the regression line of the correlation. An error of 8.1% in the calculated value compared to the actual value of the integral of Ca(t)N, could be inferred from one-point Ca(t) obtained at 6 min after the injection. Then regional cerebral blood flow was measured by the method and a significant correlation was obtained with rCBF measured using the 133Xe inhalation method (r = 0.773). The one-point Ca(t) method provides fast, easy, accurate and non-invasive measurement of rCBF without inserting catheters and without treatment of arterial blood with octanol.
我们基于微球模型，开发了一种新方法，通过单点动脉采样、使用N-异丙基-p-[123I]-碘安非他明（123I-IMP）和单光子发射计算机断层扫描（SPECT）来定量测量局部脑血流量（rCBF）。虽然传统的微球法需要持续抽取动脉血（Ca(t)的积分）并用辛醇处理血液，以获得Ca(t)积分中真正示踪剂活性的分数（N），但新方法不需要这两个步骤。我们检查了14名患者，以分析在注射123I-IMP且未进行辛醇处理后，Ca(t)N的积分与在一个时间点获得的少量动脉样本[单点Ca(t)]之间的相关性。Ca(t)N的积分是根据单点Ca(t)利用相关性的回归线计算得出的。从注射后6分钟获得的单点Ca(t)可以推断，计算值与Ca(t)N积分的实际值相比误差为8.1%。然后用该方法测量局部脑血流量，并与用133Xe吸入法测量的rCBF获得了显著相关性（r = 0.773）。单点Ca(t)法无需插入导管，也无需用辛醇处理动脉血，即可快速、简便、准确且无创地测量rCBF。
