流式细胞术在术前检测胃癌生物学变量及内镜活检获取的胃残余细胞恶性状况中的应用

Implications of flow cytometry in preoperative detection of biologic variables of gastric cancer and malignant condition of gastric remnant cells obtained by endoscopic biopsy.

作者信息

Kimura H, Kanno M, Takamura H, Arakawa H, Maeda K, Uogishi M, Sodani H, Kawashima T

机构信息

Department of Gastroenterology, Keiju Hospital, Nanao, Japan.

出版信息

Oncology. 1994 Nov-Dec;51(6):479-84. doi: 10.1159/000227389.

DOI:10.1159/000227389

PMID:7970490

Abstract

Flow cytometric (FCM) DNA studies were performed on cell suspensions obtained by endoscopic biopsy in 73 patients with gastric cancer and in 61 patients with gastric remnant. In cancer, DNA aneuploidies were detected in 49 tumors (67.1%), including 51.7% (15/29) of early gastric cancers and 77.3% (34/44) of advanced gastric cancers. Nuclear DNA content of biopsy materials had a significant correlation with that of surgically obtained ones, providing the estimation of the original ploidy pattern and DNA index (p < 0.01). The serosal invasion, lymph node metastasis, lymphatic invasion, and vascular invasion of the DNA aneuploidy were significantly higher than those of the DNA diploidy (p < 0.05). In patients with gastric remnant, 9 biopsy specimens in the anastomotic site showed DNA aneuploidy (13.9%). The DNA measurement of the endoscopically obtained specimens may provide additional preoperative information and help to identify biologic variables.

摘要

对73例胃癌患者和61例胃残端患者经内镜活检获得的细胞悬液进行了流式细胞术（FCM）DNA研究。在癌症患者中，49个肿瘤（67.1%）检测到DNA非整倍体，包括51.7%（15/29）的早期胃癌和77.3%（34/44）的进展期胃癌。活检材料的核DNA含量与手术获得材料的核DNA含量具有显著相关性，可用于估计原始倍体模式和DNA指数（p<0.01）。DNA非整倍体的浆膜侵犯、淋巴结转移、淋巴管侵犯和血管侵犯显著高于DNA二倍体（p<0.05）。在胃残端患者中，吻合部位的9份活检标本显示DNA非整倍体（13.9%）。经内镜获得标本的DNA检测可提供额外的术前信息，并有助于识别生物学变量。