Sobkowicz H M, Loftus J M, Slapnick S M
Department of Neurology, University of Wisconsin, Madison.
Acta Otolaryngol Suppl. 1993;502:3-36.
In 1975, Sobkowicz et al. (1) described long-term organotypic cultures of the organ of Corti of the newborn mouse. This paper provides detailed methods for dissection and maintenance of the isolated organ of Corti with its corresponding segment of spiral ganglion in culture. Descriptions and illustrations of cellular characteristics of the developing organ are carefully documented. The work is based on 19 years of experience and over one thousand cultures. Review of the literature and the application of the technique to research on the inner ear are provided.
1975年，索布科维茨等人（1）描述了新生小鼠柯蒂氏器的长期器官型培养。本文提供了在培养中解剖和维持分离的柯蒂氏器及其相应螺旋神经节段的详细方法。对发育中的器官的细胞特征进行了仔细记录和说明。这项工作基于19年的经验和一千多次培养。文中还提供了文献综述以及该技术在内耳研究中的应用。
