Santiago Mejia J, Nkenfou C, Southworth M W, Perler F B, Carlow C K
New England Biolabs, Beverly, MA 01915.
Parasite Immunol. 1994 Jun;16(6):297-303. doi: 10.1111/j.1365-3024.1994.tb00352.x.
In this study, the expression of an Onchocerca volvulus Ov33 homolog was demonstrated in Dirofilaria immitis. Rabbit antiserum raised against a recombinant fusion protein of O. volvulus, MBP/OvD 5B (Ov33), was found to react with a 31-33 kDa glycoprotein (DiT33) of adult worms of D. immitis. An antibody response to MBP/OvD 5B was observed in dogs, as early as 11 weeks post infection with infective larvae of D. immitis, and in dogs with occult infection. Cats both experimentally and naturally infected with D. immitis also reacted strongly with the recombinant antigen. In contrast, sera from dogs receiving chemically-abbreviated infection or from animals harbouring a variety of other helminths failed to react. These data suggest that antibody responses generated by DiT33 may have potential in immunodiagnosis of heartworm infection in cats and dogs.
在本研究中，盘尾丝虫（Onchocerca volvulus）Ov33同源物的表达在犬恶丝虫（Dirofilaria immitis）中得到证实。用盘尾丝虫的重组融合蛋白MBP/OvD 5B（Ov33）制备的兔抗血清，被发现可与犬恶丝虫成虫的一种31 - 33 kDa糖蛋白（DiT33）发生反应。在犬中，早在感染犬恶丝虫感染性幼虫后11周以及隐性感染犬中，就观察到了对MBP/OvD 5B的抗体反应。经实验和自然感染犬恶丝虫的猫也与该重组抗原有强烈反应。相比之下，接受化学简化感染的犬或携带多种其他蠕虫的动物的血清未发生反应。这些数据表明，由DiT33产生的抗体反应可能在猫和犬心丝虫感染的免疫诊断中具有潜力。
