Nail S L, Her L M, Proffitt C P, Nail L L
Department of Industrial and Physical Pharmacy, Purdue University, West Lafayette, IN 47906.
Pharm Res. 1994 Aug;11(8):1098-100. doi: 10.1023/a:1018972428858.
A microscope stage for observation of freezing and freeze drying is described. The stage uses thermoelectric (Peltier) heaters configured in two stages, with circulating fluid as a heat sink on the high temperature side. Lowest attainable sample temperature is about -47 degrees C. Principal advantages of this system are closed-loop control of stage temperature, rapid response to changes in temperature set point, and improved documentation of experiments by use of a video recorder system with a character generator which allows display of sample identity and temperature. Accuracy of measuring the sample temperature in the field of view was validated by comparing observed values of eutectic melting with published values for a series of solutes with eutectic temperatures in the range from -2 degrees C to -32 degrees C. Good agreement was obtained throughout this range.
本文描述了一种用于观察冷冻和冷冻干燥过程的显微镜载物台。该载物台采用两级配置的热电（珀耳帖）加热器，在高温侧以循环流体作为散热器。可达到的最低样品温度约为-47℃。该系统的主要优点包括载物台温度的闭环控制、对温度设定点变化的快速响应，以及通过使用带有字符发生器的视频记录系统改进实验记录，该字符发生器可显示样品标识和温度。通过将一系列共晶温度在-2℃至-32℃范围内的溶质的共晶熔化观测值与已发表的值进行比较，验证了在视野中测量样品温度的准确性。在整个范围内都获得了良好的一致性。
