Walbaum S, al Nahhas S, Gabrion C, Mesnil M, Petavy A F
INSERM Département de Parasitologie, Université Claude Bernard, Lyon, France.
Parasitol Res. 1994;80(5):381-7. doi: 10.1007/BF00932375.
Echinococcus multilocularis protoscolices collected from experimentally infected jirds were incubated for 2 weeks in rat hepatic cell cultures cocultivated with or without feeder cells (BALB/c 3T3 and IAR 20). Scanning and transmission electron microscopy studies were performed during the course of the culture period. Kupffer cells (Kc) were seen adhering to the anterior and posterior ends of the protoscolices. Some protoscolices were fixed to the cell monolayer by a cluster of Kc adhering to the posterior end of the parasite. These cells were phagocytosing the glycocalyx and the electron-dense distal end of the microthrix of the protoscolex tegument. An alteration in the superficial tegumental cytoplasm with extensive mitochondrial damage was also noted. The properties expressed by Kc against protoscolices in vitro might also be relevant for proliferation of metacestodes in vivo.
从实验感染的沙鼠体内收集的多房棘球绦虫原头节，在与有或没有饲养细胞（BALB/c 3T3和IAR 20）共培养的大鼠肝细胞培养物中孵育2周。在培养期间进行扫描和透射电子显微镜研究。可见库普弗细胞（Kc）附着于原头节的前端和后端。一些原头节通过附着在寄生虫后端的一簇Kc固定在细胞单层上。这些细胞正在吞噬原头节被膜微毛的糖萼和电子致密的远端。还注意到表面被膜细胞质发生改变，伴有广泛的线粒体损伤。Kc在体外对原头节表现出的特性可能也与体内绦虫蚴的增殖有关。
