Gabrion C, Walbaum S, al Nahhas S, Mesnil M, Petavy A F
Laboratoire de Parasitologie comparée, URA 698 CNRS, Université Montpellier II, France.
Int J Parasitol. 1995 Jan;25(1):127-30. doi: 10.1016/0020-7519(94)00086-4.
E. multilocularis protoscoleces were co-cultured with hepatic cells in the presence of IAR 20 or BALB/c 3T3 cells. Hepatocyte activity was determined by assaying transferrin and albumin secretion in culture media. The level of these 2 plasma proteins is higher in hepatic/BALB/c 3T3 co-culture medium. In the presence of parasites, the transferrin level is unchanged while the production of albumin is stimulated during the first 48 h. Our results suggest that the albumin production could be attributed to a complex cellular cooperation between hepatocytes and activated Kupffer cells as previously observed in the acute inflammatory reaction.
多房棘球绦虫原头节在IAR 20或BALB/c 3T3细胞存在的情况下与肝细胞共培养。通过检测培养基中转铁蛋白和白蛋白的分泌来测定肝细胞活性。在肝脏/BALB/c 3T3共培养基中这两种血浆蛋白的水平较高。在有寄生虫存在的情况下，转铁蛋白水平不变，而在最初48小时内白蛋白的产生受到刺激。我们的结果表明，白蛋白的产生可能归因于肝细胞与活化的库普弗细胞之间复杂的细胞合作，这与先前在急性炎症反应中观察到的情况相同。
