Miloshev G, Venkov P, van Holde K, Zlatanova J
Institute of Molecular Biology, Bulgarian Academy of Sciences, Sofia.
Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11567-70. doi: 10.1073/pnas.91.24.11567.
To elucidate the function of lysine-rich histone, yeast cells, which are believed to lack this histone, were transformed with an expression vector carrying the sea urchin histone H1 gene under control of an inducible promoter. Expression of full-length protein was tested by immunoblotting and the intracellular distribution was monitored by immunoelectron microscopy. Even low amounts of exogenous H1 led to dramatic changes in intracellular morphology and cell death. The cells that survived had lost either the plasmid or the ability to express the exogenous protein. Thus, even low amounts of canonical histone H1 are lethal to yeast cells.
为阐明富含赖氨酸组蛋白的功能，人们认为缺乏这种组蛋白的酵母细胞用一个携带在可诱导启动子控制下的海胆组蛋白H1基因的表达载体进行转化。通过免疫印迹检测全长蛋白的表达，并通过免疫电子显微镜监测细胞内分布。即使是少量的外源H1也会导致细胞内形态发生显著变化并导致细胞死亡。存活下来的细胞要么失去了质粒，要么失去了表达外源蛋白的能力。因此，即使是少量的典型组蛋白H1对酵母细胞也是致命的。
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