Knowles J A, Childs G J
Nucleic Acids Res. 1986 Oct 24;14(20):8121-33. doi: 10.1093/nar/14.20.8121.
We have isolated and sequenced a gene encoding a late H1 histone subtype from the sea urchin species L. pictus. The primary structure of the late H1 subtype encoded by this gene is 209 amino acids in length, and has a net positive charge of 67. This gene is present in a single copy per haploid genome and encodes an mRNA of 752 nucleotides. Late H1 transcripts are detected in the unfertilized egg and are most prevalent in gastrulating embryos. Comparison of 375 bp of 5' flanking sequences of the L. pictus late H1 gene and the H1-gamma gene of a distantly related sea urchin species, S. purpuratus, reveals large blocks of sequences that are identical between the two genes. To determine if these conserved 5' sequences are present in other members of the sea urchin H1 gene family, the analogous region of S. purpuratus H1-alpha, an early H1 gene, was sequenced. The homology between the flanking sequences of the early and late families was limited to consensus sequences which are found upstream of all H1 genes. The possible regulatory implications of these findings are discussed.
我们从海胆物种美西刺海胆（Lytechinus pictus）中分离并测序了一个编码晚期H1组蛋白亚型的基因。该基因编码的晚期H1亚型的一级结构长度为209个氨基酸，净正电荷为67。该基因在单倍体基因组中以单拷贝形式存在，编码一个752个核苷酸的mRNA。在未受精卵中可检测到晚期H1转录本，在原肠胚形成期的胚胎中最为普遍。对美西刺海胆晚期H1基因的375 bp 5'侧翼序列与远缘海胆物种紫球海胆（Strongylocentrotus purpuratus）的H1-γ基因进行比较，发现两个基因之间有大片相同的序列。为了确定这些保守的5'序列是否存在于海胆H1基因家族的其他成员中，对早期H1基因紫球海胆H1-α的类似区域进行了测序。早期和晚期家族侧翼序列之间的同源性仅限于在所有H1基因上游发现的共有序列。讨论了这些发现可能的调控意义。
