Sri Kantha S, Matsumura H, Kubo E, Kawase K, Takahata R, Serhan C N, Hayaishi O
Osaka Bioscience Institute, Japan.
Prostaglandins Leukot Essent Fatty Acids. 1994 Aug;51(2):87-93. doi: 10.1016/0952-3278(94)90083-3.
Prostaglandin (PG) D2 and four lipoxygenase-derived eicosanoids [lipoxins (LX) A4 and B4, and leukotrienes (LT) C4 and D4] were examined for their effects on sleep and brain temperature in freely-behaving rats. In the first series of experiments, PGD2 was infused into the third ventricle at four different locations between 23:00 and 05:00. In a location apposed to the medial preoptic area (MPO), PGD2 at doses 1, 10 and 100 pmol/min, increased the slow wave sleep (SWS) by 23% (p < or = 0.01), 35% (p < or = 0.05) and 44% (p < or = 0.01), respectively, during the infusion period. In the second series of experiments, LXs and LTs were infused at the location apposed to MPO. Significant increases in SWS were detected with LXA4 at 100 pmol/min (14%, p < or = 0.05), LXB4 at 100 pmol/min (20%, p < or = 0.05), and LTD at 10 pmol/min (17%, p < or = 0.05). An increase in paradoxical sleep (PS) was produced by PGD2 at 1 and 10 pmol/min infusion (p < or = 0.05), but not by any of the lipoxygenase-derived eicosanoids examined. PGD2 also elevated the mean brain temperature during infusion by 0.2 degrees C and 0.9 degrees C at infusion doses 10 and 100 pmol/min, respectively. But PGD2 infusion at 1 pmol/min did not elevate the brain temperature. LXs (excluding LXB4 at 100 pmol/min) and LTs did not alter the brain temperature significantly at the tested doses. We conclude that PGD2 is the most effective sleep promoter among the eicosanoids examined so far.
研究了前列腺素（PG）D2和四种脂氧合酶衍生的类二十烷酸[脂氧素（LX）A4和B4，以及白三烯（LT）C4和D4]对自由活动大鼠睡眠和脑温的影响。在第一系列实验中，于23:00至05:00之间在第三脑室的四个不同位置注入PGD2。在与内侧视前区（MPO）相对的位置，以1、10和100 pmol/分钟的剂量注入PGD2，在注入期间分别使慢波睡眠（SWS）增加23%（p≤0.01）、35%（p≤0.05）和44%（p≤0.01）。在第二系列实验中，在与MPO相对的位置注入LXs和LTs。检测到以100 pmol/分钟的剂量注入LXA4时SWS显著增加（14%，p≤0.05），以100 pmol/分钟的剂量注入LXB4时SWS显著增加（20%，p≤0.05），以10 pmol/分钟的剂量注入LTD时SWS显著增加（17%，p≤0.05）。以1和10 pmol/分钟的剂量注入PGD2会使异相睡眠（PS）增加（p≤0.05），但所检测的任何脂氧合酶衍生的类二十烷酸均未产生这种效果。PGD2在注入剂量为10和100 pmol/分钟时，还分别使注入期间的平均脑温升高0.2℃和0.9℃。但以1 pmol/分钟的剂量注入PGD2并未使脑温升高。在所测试的剂量下，LXs（不包括以100 pmol/分钟的剂量注入的LXB4）和LTs并未显著改变脑温。我们得出结论，在迄今为止所检测的类二十烷酸中，PGD2是最有效的睡眠促进剂。
