Structure, assembly and function of mammalian alpha-keto acid dehydrogenase complexes.

An attempt has been made to provide a description of the mammalian alpha-keto acid dehydrogenase multienzyme complexes, especially with respect to the structure, assembly and function of PDC and OGDC based on the investigation carried out in this laboratory over the last 14 years. Special efforts have been focused on the purification, characterization, and stoichiometric resolution into component enzymes, and the reassembling process to produce these multienzyme complexes. Studies of the macroorganization and the mechanism of regulation of these multienzyme complexes still remains a most challenging question.