Chao Y C, Wang M F, Tang H S, Hsu C T, Yin S J
Department of Internal Medicine, Tri-Service General Hospital, Taipei, Taiwan, Republic of China.
Proc Natl Sci Counc Repub China B. 1994 Jul;18(3):101-6.
We investigated the genotype of ADH2 and ADH3 in Chinese alcoholic cirrhotics and non-alcoholics by using a polymerase chain reaction and restriction-fragment-length polymorphism. This method is non-radioactive, easy to implement with good reproducibility. In the Chinese population, the frequencies of the ADH21 and ADH32 alleles were significantly higher in the alcoholic cirrhotic patients (53%; 23%) than in the viral hepatitis cirrhotics (32%; 8%) and the gastric and/or duodenal ulcer control patients (25%; 6%). On the other hand, the gastric and/or duodenal ulcer control patients and the viral hepatitis cirrhotic patients showed similar allele frequencies for the polymorphic ADH2 and ADH3 genes. These findings suggest that the alleles ADH22 and ADH31, coding for the high-Vmax beta 2-ADH and gamma 1-ADH, respectively, may play a protective role against alcoholism in Chinese patients.
我们运用聚合酶链反应和限制性片段长度多态性技术，对中国酒精性肝硬化患者和非酒精性患者的ADH2和ADH3基因型进行了研究。该方法无放射性，易于实施且重复性良好。在中国人群中，酒精性肝硬化患者中ADH21和ADH32等位基因的频率（分别为53%和23%）显著高于病毒性肝炎肝硬化患者（分别为32%和8%）以及胃和/或十二指肠溃疡对照患者（分别为25%和6%）。另一方面，胃和/或十二指肠溃疡对照患者与病毒性肝炎肝硬化患者在多态性ADH2和ADH3基因的等位基因频率上表现相似。这些发现表明，分别编码高Vmaxβ2-ADH和γ1-ADH的ADH22和ADH31等位基因，可能对中国患者的酒精成瘾起到保护作用。
