Peter B, Van Waarde M A, Vissink A, s-Gravenmade E J, Konings A W
Department of Radiobiology, University of Groningen, The Netherlands.
Radiat Res. 1994 Dec;140(3):419-28.
The aim of this study was to investigate the radiosensitivity of salivary gland tissue pretreated with isoproterenol to establish a status of depletion of secretory granules in acinar cells at the time of irradiation. Nuclear aberrations and cell lysis were taken as parameters for cell death. Local X irradiation with a single dose of 15 Gy induced comparable early morphological changes in the rat parotid and submandibular glands. During the first day after irradiation, the most obvious changes were degranulation of serous cells and induction of nuclear aberrations in both the secretory (serous as well as mucous) and intercalated duct compartment. Subsequently, progressive lysis occurred in secretory units but not in intercalated and striated ducts. Recovery of tissue integrity was observed from day 6. Early radiation-induced cell death was not reduced by isoproterenol-induced degranulation of acinar cells before irradiation. Subsequent recovery from radiation damage seemed to occur earlier in parotid glands but not in submandibular glands pretreated with isoproterenol. From the present study it is concluded that the radiosensitivity of serous salivary gland acini is not dependent on the presence of secretory granules at the time of irradiation. There was some evidence for a faster recovery from radiation damage observed after pretreatment with isoproterenol which may be the result of drug-induced stimulation of cell proliferation.
本研究的目的是调查用异丙肾上腺素预处理的唾液腺组织的放射敏感性，以确定照射时腺泡细胞中分泌颗粒的耗竭状态。核畸变和细胞裂解被用作细胞死亡的参数。单次剂量15 Gy的局部X射线照射在大鼠腮腺和下颌下腺中引起了类似的早期形态学变化。照射后的第一天，最明显的变化是浆液性细胞脱颗粒以及分泌（浆液性和黏液性）和闰管区的核畸变。随后，分泌单位发生进行性裂解，但闰管和纹状管未发生。从第6天开始观察到组织完整性的恢复。照射前异丙肾上腺素诱导的腺泡细胞脱颗粒并未减少早期辐射诱导的细胞死亡。在用异丙肾上腺素预处理的腮腺中，随后从辐射损伤中的恢复似乎更早出现，但在下颌下腺中并非如此。从本研究得出的结论是，浆液性唾液腺腺泡的放射敏感性不取决于照射时分泌颗粒的存在。有一些证据表明，用异丙肾上腺素预处理后观察到从辐射损伤中恢复得更快，这可能是药物诱导细胞增殖刺激的结果。
