辐射致大鼠颌下腺细胞凋亡及水通道蛋白 5 和转化生长因子-β表达的变化
Apoptosis and expression of AQP5 and TGF-beta in the irradiated rat submandibular gland.
机构信息
Department of Radiation Oncology, Chung-Ang University Hospital, Seoul, Korea.
出版信息
Cancer Res Treat. 2009 Sep;41(3):145-54. doi: 10.4143/crt.2009.41.3.145. Epub 2009 Sep 28.
PURPOSE
To evaluate the effect of X-ray irradiation on apoptosis and change of expression of aquaporin 5 (AQP5) and transforming growth factor-β(TGF-β) in the rat submandibular gland (SMG).
MATERIALS AND METHODS
SMGs of 120 male Sprague-Dawley rats were irradiated with a single X-ray dose (3, 10, 20, or 30 Gy). At the early and late post-irradiation phase, apoptosis was measured by the terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling (TUNEL) method, and expression of AQP5 and TGF-β was determined by immunohistochemical staining.
RESULTS
At the late post-irradiation phase, increased apoptosis was evident and marked decreases of expression of AQP5 expression by acinar cells and TGF-β expression by ductal cells were evident.
CONCLUSION
Apoptosis after X-ray irradiation develops relatively late in rat SMG. Irradiation reduces AQP5 and TGF-β expression in different SMG cell types.
目的
评估 X 射线照射对大鼠下颌下腺 (SMG) 细胞凋亡及水通道蛋白 5 (AQP5) 和转化生长因子-β (TGF-β) 表达变化的影响。
材料与方法
120 只雄性 Sprague-Dawley 大鼠的 SMG 接受单次 X 射线照射（3、10、20 或 30 Gy）。在照射后的早期和晚期，通过末端脱氧核苷酸转移酶生物素-dUTP 缺口末端标记法 (TUNEL) 测量细胞凋亡，通过免疫组织化学染色测定 AQP5 和 TGF-β 的表达。
结果
在照射后的晚期，可见明显的细胞凋亡，且腺泡细胞 AQP5 表达和导管细胞 TGF-β 表达明显减少。
结论
X 射线照射后大鼠 SMG 中的细胞凋亡发生较晚。照射会降低不同 SMG 细胞类型中 AQP5 和 TGF-β 的表达。
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