5-脂氧合酶在淋巴母细胞样B细胞和T细胞中的表达。
Expression of 5-lipoxygenase in lymphoblastoid B and T cells.
作者信息
el Makhour-Hojeij Y, Baclet M C, Chable-Rabinovitch H, Beneytout J L, Cook J
机构信息
U.R.A. CNRS 1485, Faculté de Médecine, Limoges, France.
出版信息
Prostaglandins. 1994 Jul;48(1):21-9. doi: 10.1016/0090-6980(94)90093-0.
The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-oxidoreductase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in lymphoblastoid B and T cell lines was studied at the transcriptional level by reverse transcription-PCR analysis. Two B cell lines, CESS and SKW 6.4, were found to express both 5-LOX and FLAP mRNA. One T cell line, MOLT 4, expressed only FLAP mRNA. Upon stimulation of intact cells by calcium ionophore, B lymphoblastoid cells produced 5, 12 and 15 hydroxyeicosatetraenoic acids as well as leukotriene B4.
通过逆转录 - 聚合酶链反应分析，在转录水平研究了花生四烯酸5 - 脂氧合酶（5 - LOX，花生四烯酸：氧5 - 氧化还原酶；EC 1.13.11.34）和5 - 脂氧合酶激活蛋白（FLAP）基因在淋巴母细胞性B和T细胞系中的表达。发现两个B细胞系CESS和SKW 6.4均表达5 - LOX和FLAP mRNA。一个T细胞系MOLT 4仅表达FLAP mRNA。在用钙离子载体刺激完整细胞后，B淋巴母细胞产生了5,12和15 - 羟基二十碳四烯酸以及白三烯B4。
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