脂氧合酶产物以及人培养滑膜细胞中5-脂氧合酶和5-脂氧合酶激活蛋白的表达

Lipoxygenase products and expression of 5-lipoxygenase and 5-lipoxygenase-activating protein in human cultured synovial cells.

作者信息

Bonnet C, Bertin P, Cook-Moreau J, Chable-Rabinovitch H, Treves R, Rigaud M

机构信息

Department of Rheumatology, Hôpital Dupuytren, Limoges, France.

出版信息

Prostaglandins. 1995 Sep;50(3):127-35. doi: 10.1016/0090-6980(95)00085-2.

DOI:10.1016/0090-6980(95)00085-2

PMID:8750209

Abstract

5-Lipoxygenase products are pro-inflammatory mediators. Their roles and cellular origin in chronic inflammatory rheumatisms such as rheumatoid arthritis (RA) are poorly understood. The expression of arachidonate 5-lipoxygenase (5-LOX, arachidonate: oxygen 5-oxydoreductase; EC 1.13.11.34) and the 5-lipoxygenase activating protein (FLAP) genes in osteoarthritis and RA synoviocytes was studied at the transcriptional level by reverse transcription-polymerase chain reaction (RT-PCR) methodology. Arachidonic acid metabolism was analyzed by reverse-phase high pressure liquid chromatography. 5-LOX and FLAP mRNA were detectable using RT-PCR in all sources of synoviocytes tested. The expression of 5-LOX and FLAP mRNA led to the synthesis of 5-LOX metabolites. 12- and 15-LOX activities were also present. These LOX products can participate in inflammatory processes leading to joint destruction in RA.

摘要

5-脂氧合酶产物是促炎介质。它们在类风湿关节炎（RA）等慢性炎症性风湿病中的作用和细胞来源尚不清楚。采用逆转录-聚合酶链反应（RT-PCR）方法，在转录水平研究了骨关节炎和RA滑膜细胞中花生四烯酸5-脂氧合酶（5-LOX，花生四烯酸：氧5-氧化还原酶；EC 1.13.11.34）和5-脂氧合酶激活蛋白（FLAP）基因的表达。通过反相高压液相色谱分析花生四烯酸代谢。使用RT-PCR在所有测试的滑膜细胞来源中均可检测到5-LOX和FLAP mRNA。5-LOX和FLAP mRNA的表达导致了5-LOX代谢产物的合成。同时也存在12-和15-脂氧合酶活性。这些脂氧合酶产物可参与导致RA关节破坏的炎症过程。