大鼠前列腺素F2α受体cDNA的克隆与表达
Cloning and expression of a cDNA for rat prostaglandin F2 alpha receptor.
作者信息
Kitanaka J, Hasimoto H, Sugimoto Y, Negishi M, Aino H, Gotoh M, Ichikawa A, Baba A
机构信息
Department of Pharmacology, Faculty of Pharmaceutical Sciences, Osaka University, Japan.
出版信息
Prostaglandins. 1994 Jul;48(1):31-41. doi: 10.1016/0090-6980(94)90094-9.
We have cloned a cDNA for rat prostaglandin (PG) F2 alpha receptor from cultured rat astrocytes. The cDNA encodes a polypeptide of 366 amino acids with seven putative transmembrane domains. Specific binding of [3H]PGF2 alpha in membranes of COS-7 cells transfected with the cDNA was displaced with unlabeled PGs in the order of PGF2 alpha > PGD2 > PGE2 > PGI2. In the cDNA-transfected LLC-PK1 cells, PGF2 alpha stimulated phosphoinositide hydrolysis. A significant 4.7-kb mRNA transcript was detected in cultured rat astrocytes and whole brain and pregnant ovary of adult rats by Northern blot analysis.
我们从培养的大鼠星形胶质细胞中克隆了大鼠前列腺素（PG）F2α受体的cDNA。该cDNA编码一个含有366个氨基酸的多肽，具有七个推定的跨膜结构域。用该cDNA转染的COS-7细胞膜中[3H]PGF2α的特异性结合被未标记的PGs以PGF2α>PGD2>PGE2>PGI2的顺序取代。在cDNA转染的LLC-PK1细胞中，PGF2α刺激了磷酸肌醇水解。通过Northern印迹分析在培养的大鼠星形胶质细胞、成年大鼠的全脑和妊娠卵巢中检测到一个显著的4.7-kb mRNA转录本。
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