Sando T, Usui T, Tanaka I, Mori K, Sasaki Y, Fukuda Y, Namba T, Sugimoto Y, Ichikawa A, Narumiya S
Department of Medicine, Kyoto University Faculty of Medicine, Japan.
Biochem Biophys Res Commun. 1994 May 16;200(3):1329-33. doi: 10.1006/bbrc.1994.1596.
A cDNA clone encoding the rat prostaglandin (PG) E receptor EP2 subtype was cloned from a rat lung cDNA library. It encodes 488 amino acid residues with putative seven-transmembrane domains. Specific binding of [3H]PGE2 was found in COS-7 cells transfected with the cDNA and was displaced with unlabeled prostaglandins in the order of PGE2 = PGE1 >> iloprost > or = PGF2 alpha > or = PGD2. The binding was also inhibited by misoprostol, an EP2 and EP3 agonist, but not by sulprostone, an EP1 and EP3 agonist. Northern blot analysis demonstrated that the EP2 mRNA is widely expressed in various tissues, the significant expression being observed in the thymus, lung, spleen, heart stomach, and pancreas.
从大鼠肺cDNA文库中克隆出一个编码大鼠前列腺素（PG）E受体EP2亚型的cDNA克隆。它编码488个氨基酸残基，具有推定的七个跨膜结构域。在用该cDNA转染的COS-7细胞中发现了[3H]PGE2的特异性结合，并且未标记的前列腺素按PGE2 = PGE1 >> 依洛前列素 ≥ PGF2α ≥ PGD2的顺序取代了这种结合。这种结合也被米索前列醇（一种EP2和EP3激动剂）抑制，但不被舒前列素（一种EP1和EP3激动剂）抑制。Northern印迹分析表明，EP2 mRNA在各种组织中广泛表达，在胸腺、肺、脾、心脏、胃和胰腺中观察到显著表达。
