Kaushal S, Schneider J W, Nadal-Ginard B, Mahdavi V
Department of Cardiology, Children's Hospital, Harvard Medical School, Boston, MA 02115.
Science. 1994 Nov 18;266(5188):1236-40. doi: 10.1126/science.7973707.
Muscle enhancer factor-2A (MEF2A), a member of the MADS family, induced myogenic development when ectopically expressed in clones of nonmuscle cells of human clones, a function previously limited to the muscle basic helix-loop-helix (bHLH) proteins. During myogenesis, MEF2A and bHLH proteins cooperatively activate skeletal muscle genes and physically interact through the MADS domain of MEF2A and the three myogenic amino acids of the muscle bHLH proteins. Thus, skeletal myogenesis is mediated by two distinct families of mutually inducible and interactive muscle transcription factors, either of which can initiate the developmental cascade.
肌肉增强因子2A（MEF2A）是MADS家族的成员之一，当在人类克隆的非肌肉细胞克隆中异位表达时，它可诱导肌源性发育，这一功能以前仅限于肌肉碱性螺旋-环-螺旋（bHLH）蛋白。在肌生成过程中，MEF2A和bHLH蛋白协同激活骨骼肌基因，并通过MEF2A的MADS结构域与肌肉bHLH蛋白的三个肌源性氨基酸进行物理相互作用。因此，骨骼肌生成由两个不同的、相互诱导和相互作用的肌肉转录因子家族介导，其中任何一个都可以启动发育级联反应。
