Gu J F, Shi Y L
Shanghai Institute of Physiology, Chinese Academy of Sciences.
Sheng Li Xue Bao. 1994 Jun;46(3):244-8.
The neuroblastoma x glioma hybrid clone NG108-15 which were derived by fusion of mouse neuroblastoma clone N18TG6 with rat glioma clone C6BU1 proliferates in the presence of fetal calf serum, but differentiates when intracellular cAMP concentration is made to increase by addition of dBcAMP to the cultural medium. After differentiation, the cells express many neural properties. In this experiment, by means of an automatic colorimetric microassay for quantifying the growth and Brilliant Cresyl Violett stain for revealing Nissl substance, the effect of hypoxia (2% O2) on the hybrid cells was investigated. The results obtained were as follows: (1) Hypoxia depressed proliferation of non-differentiated cells and induced death of differentiated ones. (2) Hypoxia inhibited differentiation, either non-differentiated or differentiating cells, in the direction to be a neuro-like cell: some cells became large flat with short processes and had no Nissl substance. Whether hypoxia would enable non-differentiated NG108-15 cells to differentiate in a direction to express more properties of glial cell is an interesting problem.
神经母细胞瘤x胶质瘤杂交克隆NG108 - 15是由小鼠神经母细胞瘤克隆N18TG6与大鼠胶质瘤克隆C6BU1融合得到的，它在胎牛血清存在的情况下增殖，但当通过向培养基中添加二丁酰环磷腺苷（dBcAMP）使细胞内cAMP浓度升高时会发生分化。分化后，细胞表达多种神经特性。在本实验中，通过自动比色微量测定法来定量细胞生长，并采用灿烂甲酚紫染色法来显示尼氏物质，研究了缺氧（2%氧气）对杂交细胞的影响。得到的结果如下：（1）缺氧抑制未分化细胞的增殖，并诱导分化细胞死亡。（2）缺氧抑制未分化或正在分化的细胞向神经样细胞方向分化：一些细胞变得又大又平，有短突起，且没有尼氏物质。缺氧是否会使未分化的NG108 - 15细胞向表达更多神经胶质细胞特性的方向分化是一个有趣的问题。
