Yu J, Tong S, Qi J, Kao F T
Eleanor Roosevelt Institute for Cancer Research, Denver, Colorado 80206.
Somat Cell Mol Genet. 1994 Jul;20(4):353-7. doi: 10.1007/BF02254724.
The short arm of human chromosome 2, comprising approximately 93 million bp, has been divided into four regions to construct region-specific microdissection libraries to facilitate physical mapping and gene cloning. These four regions include 2p23-p25 (designated 2P1), 2p21-p23 (2P2), 2p14-p16 (2P3), and 2p11-p13 (2P4). Together with three previously constructed microdissection libraries of 2P1, 2P2 and 2P4, a fourth library for the region 2p14-p16 (2P3) has been constructed and characterized to complete all four region-specific libraries for the entire 2p. The 2P3 library is very large, potentially comprising 1,000,000 recombinant microclones with insert sizes ranging between 50 and 800 bp and a mean of 250 bp. Approximately 40% of the microclones contain unique sequences. Of the 77 single-copy microclones analyzed, 66 clones (86%) hybridized to both human and chromosome 2 DNAs, indicating that they were derived from human and are chromosome 2 specific. The hybridizing HindIII genomic fragments for the 66 microclones have also been determined.
人类2号染色体的短臂约含9300万个碱基对，已被划分为四个区域，用于构建区域特异性显微切割文库，以促进物理图谱绘制和基因克隆。这四个区域包括2p23 - p25（命名为2P1）、2p21 - p23（2P2）、2p14 - p16（2P3）和2p11 - p13（2P4）。连同之前构建的2P1、2P2和2P4的三个显微切割文库，已构建并鉴定了2p14 - p16区域（2P3）的第四个文库，以完成整个2p的所有四个区域特异性文库。2P3文库非常大，可能包含100万个重组微克隆，插入片段大小在50至800碱基对之间，平均为250碱基对。大约40%的微克隆含有独特序列。在分析的77个单拷贝微克隆中，66个克隆（86%）与人DNA和2号染色体DNA都杂交，表明它们源自人类且具有2号染色体特异性。还确定了这66个微克隆的杂交HindIII基因组片段。
