Click R E, Weisberg D, White J, Reichenbach D, Jamieson S W
Department of Biology, University of Wisconsin, River Falls 54022.
Transplantation. 1994 Nov 15;58(9):1020-6. doi: 10.1097/00007890-199411150-00008.
Although xenografts can be rejected by humoral or cellular mechanisms, the relative contribution of each remains unknown for any given recipient-donor combination. Moreover, the cells involved in cell-mediated events, as well as the mechanisms by which these cells recognize xenoantigens, remain controversial. It would be advantageous to have a model in which either, as well as various parts of either, could be investigated in the absence of the other, as well as in the absence of events taking place during organ engraftment. In the present report, rejection of rat skin xenografts was monitored after adoptive transfer of unique populations of highly purified lymphoid cells to previously transplanted immunodeficient C.B17 Scid/Scid mice. Purified T cells and, unexpectedly, purified CD4+ T cells alone and purified CD8+ T cells alone rejected rat xenografts. Alternatives that may explain these findings are discussed.
虽然异种移植可通过体液或细胞机制被排斥，但对于任何给定的受体 - 供体组合，每种机制的相对贡献仍不清楚。此外，参与细胞介导事件的细胞以及这些细胞识别异种抗原的机制仍存在争议。拥有一个模型将是有利的，在该模型中，可以在不存在另一方的情况下，以及在不存在器官移植过程中发生的事件的情况下，对其中任何一方以及任何一方的各个部分进行研究。在本报告中，将高度纯化的独特淋巴细胞群体过继转移到先前移植的免疫缺陷C.B17 Scid/Scid小鼠后，监测大鼠皮肤异种移植的排斥情况。纯化的T细胞，以及出乎意料的单独的纯化CD4 + T细胞和单独的纯化CD8 + T细胞均可排斥大鼠异种移植物。文中讨论了可能解释这些发现的其他情况。
