Pathak K M, Allan J C, Ersfeld K, Craig P S
Department of Parasitology, College of Veterinary and Animal Sciences, Rajasthan, India.
Vet Parasitol. 1994 Jun;53(3-4):209-17. doi: 10.1016/0304-4017(94)90184-8.
An enzyme-linked immunoelectrotransfer blot (EITB) was developed using soluble Taenia solium metacestode antigen preparation for immunodiagnosis of porcine cysticercosis and compared with an enzyme-linked immunosorbent assay (ELISA). Sera from 20 pigs with parasitologically confirmed cysticercosis were tested by both EITB and ELISA assays. Specificity of the tests was examined by testing 25 serum samples from the UK, where cysticercosis is not endemic and sera from pigs with Echinococcus granulosus (15), Fasciolopsis buski (six), or Trichinella spiralis (five) infections. All but two of the samples from cases of confirmed cysticercosis were positive by EITB and none of the samples from healthy controls or heterologous infections reacted to any of the diagnostic bands. Thus, the test was 90% sensitive and 100% specific. The sensitivity of the ELISA was 70% with 73% specificity, cross-reactions occurring with sera from E. granulosus infected pigs. Four polypeptides (8, 11, 16 and 23 kDa) were identified by SDS-PAGE and EITB that were specifically recognized by pigs with confirmed cysticercosis.
利用猪带绦虫囊尾蚴可溶性抗原制备物开发了一种酶联免疫电转移印迹法（EITB），用于猪囊尾蚴病的免疫诊断，并与酶联免疫吸附测定法（ELISA）进行比较。用EITB和ELISA检测了20头经寄生虫学确诊为囊尾蚴病的猪的血清。通过检测来自英国的25份血清样本（英国并非囊尾蚴病的流行地区）以及感染细粒棘球绦虫（15份）、姜片吸虫（6份）或旋毛虫（5份）的猪的血清来检验检测方法的特异性。经确诊为囊尾蚴病病例的样本中，除两份外，其余所有样本经EITB检测均呈阳性，健康对照或异种感染的样本对任何诊断条带均无反应。因此，该检测方法的灵敏度为90%，特异性为100%。ELISA的灵敏度为70%，特异性为73%，感染细粒棘球绦虫的猪的血清会出现交叉反应。通过十二烷基硫酸钠 - 聚丙烯酰胺凝胶电泳（SDS - PAGE）和EITB鉴定出4种多肽（8、11、16和23 kDa），它们能被经确诊为囊尾蚴病的猪特异性识别。
