Gupta K K, Singh A, Singh A K, Singh S K, Tripathi M, Gupta R K, Prasad K N
Department of Microbiology,Sanjay Gandhi Postgraduate Institute of Medical Sciences,Lucknow,India.
Department of Immunology,Weizmann Institute of Science,Rehovot,Israel.
J Helminthol. 2019 Sep;93(5):548-551. doi: 10.1017/S0022149X18000603. Epub 2018 Jul 16.
Taenia solium cysticercosis is a major public health problem in developing countries. Swine cysticercosis results in economic losses for pig farmers in disease endemic areas. Consumption of cysticercotic pork leads to taeniasis in humans. Eggs excreted in the faeces of T. solium carriers disseminate to humans and pigs through the faecal-oral route, thus maintaining the life cycle in endemic areas. An enzyme-linked immunoelectrotransfer blot (EITB) assay was developed using whole crude T. solium cysticercus antigens (WCA) for the diagnosis of swine cysticercosis. Sera from 30 swine with cysticercosis confirmed by magnetic resonance imaging were subjected to EITB assay. Sera from 50 swine that were raised in a government farm and not allowed to roam freely were included as negative controls. Two or more bands of 8, 11, 14, 24, 26 and 29 kDa were immunoreactive on blot with sera from all infected swine except two, and none from swine raised on the government farm. The overall sensitivity and specificity of EITB assay for diagnosis of swine cysticercosis were 93.3% and 100%, respectively. Hence, EITB assay based on WCA may be a suitable diagnostic tool for swine cysticercosis in endemic areas.
猪带绦虫囊尾蚴病是发展中国家的一个主要公共卫生问题。猪囊尾蚴病给疾病流行地区的养猪户造成经济损失。食用含有囊尾蚴的猪肉会导致人类患绦虫病。猪带绦虫携带者粪便中排出的虫卵通过粪-口途径传播给人类和猪，从而在流行地区维持生命周期。利用猪带绦虫囊尾蚴全粗抗原（WCA）开发了一种酶联免疫电转移印迹（EITB）试验，用于诊断猪囊尾蚴病。对30头经磁共振成像确诊患有囊尾蚴病的猪的血清进行EITB试验。将50头在政府农场饲养且不允许自由放养的猪的血清作为阴性对照。除两头猪外，所有感染猪的血清在印迹上与8、11、14、24、26和29 kDa的两条或更多条带发生免疫反应，而政府农场饲养的猪的血清则无反应。EITB试验诊断猪囊尾蚴病的总体敏感性和特异性分别为93.3%和100%。因此，基于WCA的EITB试验可能是流行地区猪囊尾蚴病的一种合适诊断工具。
