Gatewood D M, Fenwick B W, Chengappa M M
Department of Laboratory Medicine, Kansas State University, Manhattan 66506.
Vet Microbiol. 1994 Aug 1;41(3):221-33. doi: 10.1016/0378-1135(94)90103-1.
Pasteurella haemolytica, strain P1148 (biotype A, serotype 1) was grown under iron-rich and iron-restricted conditions both with and without serum, and the outer membrane protein (OMP), capsule, and leukotoxin production studied. OMPs were evaluated by SDS-PAGE and examined by immunoblot to identify antigens recognized by sera from P. haemolytica A1 convalescent and vaccinated cattle. Capsule production was evaluated using fluorescent antibody staining and rapid plate agglutination reaction. Leukotoxin production was measured by neutrophil 51Cr-release assay. Expression of specific OMPs, amount and antigenic character of capsule, and quantity of leukotoxin produced by P. haemolytica A1 varied in response to alterations in the growth media. Immunoblots indicated the immune response of convalescent calves differs from vaccinated calves, and convalescent calves produce antibodies to novel OMPs induced by growth in iron-restricted conditions.
溶血巴斯德氏菌P1148菌株（生物型A，血清型1）在有血清和无血清的富铁及缺铁条件下培养，并对其外膜蛋白（OMP）、荚膜和白细胞毒素的产生进行了研究。通过SDS-PAGE评估OMP，并通过免疫印迹法检测，以鉴定溶血巴斯德氏菌A1康复牛和接种疫苗牛血清所识别的抗原。使用荧光抗体染色和快速平板凝集反应评估荚膜的产生。通过中性粒细胞51Cr释放试验测定白细胞毒素的产生。溶血巴斯德氏菌A1产生的特定OMP的表达、荚膜的数量和抗原特性以及白细胞毒素的数量因生长培养基的变化而有所不同。免疫印迹表明，康复犊牛的免疫反应与接种疫苗的犊牛不同，康复犊牛会产生针对缺铁条件下生长诱导产生的新型OMP的抗体。
