Confer A W, McCraw R D, Durham J A, Morton R J, Panciera R J
Department of Veterinary Pathology, Oklahoma State University, Stillwater 74078, USA.
Vet Immunol Immunopathol. 1995 Jul;47(1-2):101-10. doi: 10.1016/0165-2427(94)05390-e.
Serum antibody responses to the 70, 77, and 100 kDa iron-regulated outer membrane proteins (IROMPs) of Pasteurella haemolytica A1 were studied in cattle vaccinated with outer membrane protein (OMP) enriched outer membrane fraction, IROMP-enriched outer membrane fraction or live P. haemolytica. Vaccination with an IROMP-enriched outer membrane fraction stimulated antibodies to the 70 kDa IROMP, whereas vaccination with live P. haemolytica stimulated antibodies to the 70 and 77 kDa IROMPs. In a second experiment, sera were used from cattle vaccinated with live or killed P. haemolytica and subsequently challenged. Significant antibody responses to OMP- and IROMP-enriched outer membrane fractions were detected by an enzyme-linked immunosorbent assay (ELISA) for cattle vaccinated with bacterins or live P. haemolytica. Regression analysis indicated significant correlations between high antibody responses to the OMP- or IROMP-enriched fraction and resistance to challenge. Antibody responses to the 70 and 77 kDa IROMPs were significantly greater for the live P. haemolytica vaccinates than for PBS control vaccinates. There was no significant correlation between antibody responses to individual IROMPs and resistance or susceptibility to challenge. These data suggest that antibodies to IROMPs alone are probably not responsible for protective immunity against pneumonic pasteurellosis. Antibodies to IROMPs, however, in conjunction with antibodies to other surface antigens probably enhance immunity to P. haemolytica challenge.
研究了用富含外膜蛋白（OMP）的外膜组分、富含铁调节外膜蛋白（IROMP）的外膜组分或溶血巴斯德菌活菌对牛进行疫苗接种后，牛血清对溶血巴斯德菌A1的70、77和100 kDa铁调节外膜蛋白（IROMP）的抗体反应。用富含IROMP的外膜组分进行疫苗接种可刺激产生针对70 kDa IROMP的抗体，而用溶血巴斯德菌活菌进行疫苗接种则刺激产生针对70和77 kDa IROMP的抗体。在第二个实验中，使用了接种过溶血巴斯德菌活菌或灭活菌并随后受到攻击的牛的血清。通过酶联免疫吸附测定（ELISA）检测到，接种灭活疫苗或溶血巴斯德菌活菌的牛对富含OMP和IROMP的外膜组分有显著的抗体反应。回归分析表明，对富含OMP或IROMP的组分的高抗体反应与抗攻击能力之间存在显著相关性。接种溶血巴斯德菌活菌的牛对70和77 kDa IROMP的抗体反应明显高于接种PBS对照的牛。对单个IROMP的抗体反应与抗攻击能力或易感性之间没有显著相关性。这些数据表明，单独针对IROMP的抗体可能不是抗肺炎巴氏杆菌病保护性免疫的原因。然而，针对IROMP的抗体与针对其他表面抗原的抗体结合，可能会增强对溶血巴斯德菌攻击的免疫力。
