Rabouille A, Bigot Y, Drezen J M, Sizaret P Y, Hamelin M H, Periquet G
Institut de Biocénotique Expérimentale des Agrosystèmes, Faculté des Sciences, Tours, France.
Virology. 1994 Nov 15;205(1):228-37. doi: 10.1006/viro.1994.1638.
Wild and laboratory populations of the parasitoid wasp Diadromus pulchellus are infected with a new member of the reoviridae (DpRV) described in this paper. The particles of this virus possess two capsid shells (diameters: 35 and 70 nm) made up of 11 proteins. The virus is present mainly in the gut of the wasp, with smaller quantities in its venom gland. The genome of virus particles purified from haploid insects (functional males) contains 10 segments, whereas virus from diploid insects (females and sterile diploid males) contains a supernumerary 3.33-kb segment. The sequence of this dsRNA segment revealed that it is a triplicated 1050-bp motif which is 97.5% similar to the 5' region of one of the 10 basic segments, the 3.80-kb segment.
本文描述了一种新的呼肠孤病毒(DpRV),该病毒感染了野生和实验室种群的拟寄生物黄蜂Diadromus pulchellus。这种病毒的颗粒具有由11种蛋白质组成的两层衣壳(直径分别为35纳米和70纳米)。该病毒主要存在于黄蜂的肠道中,在其毒腺中的含量较少。从单倍体昆虫(功能性雄性)中纯化的病毒颗粒基因组包含10个片段,而来自二倍体昆虫(雌性和不育二倍体雄性)的病毒则包含一个额外的3.33 kb片段。该双链RNA片段的序列显示,它是一个重复三次的1050 bp基序,与10个基本片段之一的3.80 kb片段的5'区域相似度为97.5%。