Svechnikov K V, Busygina T V, Osadchuk A V
Vopr Med Khim. 1994 Jul-Aug;40(4):14-7.
A very effective procedure was developed for separation of sex hormones of the delta 4 pathway using the microcolumn technique of high performance liquid chromatography (HPLC). A five-step gradient containing methanol, acetonitrile and water was used as a mobile phase, while Lychrosorb RP-18 with particles of 5 microns was used as a sorbent. Metabolism of steroid hormones and activity of 3 beta-steroid dehydrogenase, 17 alpha-hydroxylase, C17-20-lyase and 17-ketosteroid reductase were studied in Leydig cells of two mouse strains PT and A/He. The higher rate of steroid metabolism and of the enzymatic activity was detected in the mouse cells of PT strain as compared with those of A/He strain.
采用高效液相色谱（HPLC）微柱技术开发了一种非常有效的程序，用于分离δ4途径的性激素。使用含有甲醇、乙腈和水的五步梯度作为流动相，而使用粒径为5微米的Lychrosorb RP-18作为吸附剂。在两种小鼠品系PT和A/He的睾丸间质细胞中研究了类固醇激素的代谢以及3β-类固醇脱氢酶、17α-羟化酶、C17-20裂解酶和17-酮类固醇还原酶的活性。与A/He品系的小鼠细胞相比，在PT品系的小鼠细胞中检测到更高的类固醇代谢率和酶活性。
