Osadchuk A V, Svechnikov K V
Institute of Cytology and Genetics, Siberian Division, Russian Academy of Sciences, Novosibirsk, Russia.
Genetika. 1998 Sep;34(9):1277-85.
Four activities of key microsomal steroidogenic enzymes (3 beta-hydroxysteroid dehydrogenase-isomerase, microsomal cytochrome P45017 beta, and 17-ketosteroid reductase) were compared in Leydig cells of six inbred strains of mice: A/He, CBA/Lac, C57BL/6J, DD, YT, and PT. The activities of the enzymes were found to vary considerably from one strain to another, depending on the genotype. Analysis of testosterone biosynthesis revealed a decreased activity of 17-ketosteroid reductase as compared to the activities of other microsomal steroidogenic enzymes. The metabolism of pregnenolone and progesterone demonstrated features dependent on the genotype and caused by genotypic differences in the activities of microsomal steroidogenic enzymes. These activities demonstrated a correlative interstrain variation indicative of their coordinated genetic control. The correlative variation may be related to a major gene effect on the activities of microsomal steroidogenic enzymes.
在六种近交系小鼠（A/He、CBA/Lac、C57BL/6J、DD、YT和PT）的睾丸间质细胞中，比较了关键微粒体类固醇生成酶（3β-羟基类固醇脱氢酶-异构酶、微粒体细胞色素P45017β和17-酮类固醇还原酶）的四种活性。发现这些酶的活性因菌株而异，取决于基因型。睾酮生物合成分析显示，与其他微粒体类固醇生成酶的活性相比，17-酮类固醇还原酶的活性降低。孕烯醇酮和孕酮的代谢表现出依赖于基因型的特征，这些特征是由微粒体类固醇生成酶活性的基因型差异引起的。这些活性表现出种间相关变异，表明它们受到协调的遗传控制。这种相关变异可能与一个主要基因对微粒体类固醇生成酶活性的影响有关。
