Vasil'eva S M, Muliavko N A, Donchenko G V
Vopr Med Khim. 1994 Jul-Aug;40(4):8-11.
Dynamics of the DNA main reparative reactions was studied in rat liver tissue after N-methyl-N-nitrose urea treatment and simultaneous stimulation of NAD biosynthesis in order to evaluate the interrelationship between DNA repair and poly-ADP-ribosylation. Treatment of liver tissue with the mutagen was followed by the preparations incubation in Hanks solution within 30 min at 37 degrees. During the initial steps of restoration an increase in amount of DNA breaks proved to occur in liver tissue of the experimental animals as compared with controls; these deteriorations were decreased down to initial level to the final steps of incubation, while content of breaks in single-strand DNA remained to be high in liver tissue of control animals. The intensive rate of DNA repair in rat liver tissue containing high level of NAD correlated with an increase in nuclear endonuclease and DNA-dependent-DNA-beta-polymerase activities. At the same time, the activity of poly-ADP-ribosyl polymerase was only slightly altered during all the steps of the tissue repair studied.
为了评估DNA修复与多聚ADP核糖基化之间的相互关系，研究了N-甲基-N-亚硝基脲处理大鼠肝脏组织并同时刺激NAD生物合成后DNA主要修复反应的动力学。用诱变剂处理肝脏组织后，将制剂在37℃的汉克斯溶液中孵育30分钟。在恢复的初始阶段，与对照组相比，实验动物肝脏组织中DNA断裂数量增加；到孵育的最后阶段，这些损伤减少到初始水平，而对照动物肝脏组织中单链DNA的断裂含量仍然很高。大鼠肝脏组织中DNA修复的强烈速率与高水平NAD时核内切核酸酶和DNA依赖性DNA-β聚合酶活性的增加相关。同时，在研究的组织修复的所有步骤中，多聚ADP核糖基聚合酶的活性仅略有改变。
