非洲马瘟病毒第10节段及其他环状病毒同源基因的系统发育分析。
Phylogenetic analysis of segment 10 from African horsesickness virus and cognate genes from other orbiviruses.
作者信息
de Sá R O, Zellner M, Grubman M J
机构信息
Department of Biology, University of Richmond, VA 23173.
出版信息
Virus Res. 1994 Aug;33(2):157-65. doi: 10.1016/0168-1702(94)90052-3.
Utilizing the reverse transcriptase-polymerase chain reaction (RT-PCR) procedure, we have synthesized full-length copies of segment 10 from African horsesickness virus (AHSV) serotypes 1, 4 and 8. The genes were cloned, sequenced and compared with the sequence of the cognate gene from AHSV serotypes 3 and 9. Sequences were analyzed to assess evolutionary relationships among serotypes using cladistics. Based on this analysis the data support a close relationship between serotypes 4 and 9 and between serotypes 1 and 8 and a closer relationship of serotype 3 to the 4 and 9 group.
利用逆转录聚合酶链反应（RT-PCR）技术，我们合成了非洲马瘟病毒（AHSV）血清型1、4和8的第10节段的全长拷贝。对这些基因进行了克隆、测序，并与AHSV血清型3和9的同源基因序列进行了比较。使用分支系统学分析序列，以评估血清型之间的进化关系。基于该分析，数据支持血清型4和9之间、血清型1和8之间存在密切关系，以及血清型3与血清型4和9组之间存在更密切的关系。
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