Inoue O, Kobayashi K
Division of Clinical Research and Radiation Health, National Institute of Radiological Sciences, Chiba, Japan.
Nihon Shinkei Seishin Yakurigaku Zasshi. 1994 Aug;14(4):215-25.
In vivo receptor mapping in human brain with positron emission tomography (PET) and single-photon emission tomography (SPECT) is of great value for neuropharmacology and psychiatry. However, it is unclear yet the characteristics of receptor binding in vivo, and significant discrepancies of receptor binding between in vitro and in vivo have been observed. It seems to be important to clarify the characteristics of receptor binding in vivo as compared with in vitro binding systems. In this review, procedures of receptor binding studies using small animals and 3H-labeled ligands, methodological problem as well as the kinetic analysis method are discussed.
利用正电子发射断层扫描（PET）和单光子发射断层扫描（SPECT）对人脑进行体内受体图谱研究，对神经药理学和精神病学具有重要价值。然而，体内受体结合的特征尚不清楚，并且已经观察到体外和体内受体结合存在显著差异。与体外结合系统相比，阐明体内受体结合的特征似乎很重要。在这篇综述中，讨论了使用小动物和3H标记配体进行受体结合研究的程序、方法学问题以及动力学分析方法。
