Zhao D B, Wu Y L, Yu H, Guo L H, Hong L S, Wang Y H
Shanghai Institute of Cell Biology, Chinese Acedemy of Sciences.
Shi Yan Sheng Wu Xue Bao. 1994 Jun;27(2):215-23.
Zip gene is required during the late neurogenesis of Drosophila. Molecular analysis of zip gene revealed that it encodes an integral membrane glycoprotein, functioning as a cellular recognition and/or adhesion molecule. It has been cloned into an expression plasmid pWR 590. By expression of this plasmid in E. coli, a fusion protein of zip with lacZ has been purified and used to prepare polyclonal antibodies from rabbits. After being identified by West blotting, antibodies were used to react with the whole mount embryos of Drosophila. The results of this antibody labelling experiment showed that zip proteins are fundamentally synthesized after germ band shortening, supporting our previous predication that zip gene is involved in the late neurogenesis and that antibodies against zip recognize lateral neural fascicles as well as neurons in CNS, proving that zip protein expressed in CNS may play a role in the establishment and maintenance of neural fasciculation.
Zip基因在果蝇神经发生后期是必需的。对Zip基因的分子分析表明，它编码一种整合膜糖蛋白，作为一种细胞识别和/或粘附分子发挥作用。它已被克隆到表达质粒pWR 590中。通过在大肠杆菌中表达该质粒，纯化了Zip与lacZ的融合蛋白，并用于从兔制备多克隆抗体。经蛋白质免疫印迹鉴定后，抗体用于与果蝇的整体胚胎反应。该抗体标记实验的结果表明，Zip蛋白基本上是在胚带缩短后合成的，支持了我们之前的预测，即Zip基因参与神经发生后期，并且针对Zip的抗体识别中枢神经系统中的外侧神经束以及神经元，证明在中枢神经系统中表达的Zip蛋白可能在神经束的建立和维持中发挥作用。
