Nakayama T, Kudo H, Suzuki S, Suenaga M, Sakamoto S
School of Allied Health Sciences, Faculty of Medicine, Tokyo Medical and Dental University, Japan.
Acta Haematol. 1994;91(4):194-8. doi: 10.1159/000204333.
In an attempt to define the effects of recombinant human erythropoietin (rEPO) on DNA synthesis in hematopoietic organs, we investigated DNA-synthesizing enzyme activities, i.e., thymidylate synthetase and thymidine kinase activities, and bromodeoxyuridine-immunohistochemistry during the recovery phase of hematopoietic cells in bone marrow and spleen after the hypoplastic period induced by cyclophosphamide (Cy) treatment in rats. Treatment with rEPO increased enzyme activities and cell number of erythroid series in bone marrow cells; it also increased organ weight and S-phase cells in the spleen, followed by an augmentation of the number of erythrocytes and a rise in the hemoglobin and hematocrit levels in peripheral blood with or without Cy treatment.
为了确定重组人促红细胞生成素（rEPO）对造血器官中DNA合成的影响，我们在大鼠经环磷酰胺（Cy）处理诱导造血细胞发育不全后，对骨髓和脾脏中造血细胞恢复阶段的DNA合成酶活性，即胸苷酸合成酶和胸苷激酶活性，以及溴脱氧尿苷免疫组织化学进行了研究。rEPO治疗增加了骨髓细胞中红系的酶活性和细胞数量；它还增加了脾脏的器官重量和S期细胞数量，随后无论是否进行Cy治疗，外周血中的红细胞数量均增加，血红蛋白和血细胞比容水平也升高。
