Kudo H, Sakamoto S, Suzuki S, Nakayama T, Suenaga M, Tomiyama J, Adachi Y
Division of Blood Transfusion, Tokyo Metropolitan Bokuto General Hospital, Japan.
In Vivo. 1994 May-Jun;8(3):303-8.
In an attempt to define a regulation of pyrimidine nucleotide synthesis in hematopoietic cells, we investigated the activities of thymidylate synthetase and thymidine kinase, and the cell cycle by flow cytometric DNA analysis and bromodeoxyuridine-immunohistochemistry during the recovery phase of hematopoietic cells in bone marrow after the hypoplastic period induced by cyclophosphamide treatment in rats. The hematopoietic cells first entered into cell cycle via the de novo pathway in pyrimidine nucleotide synthesis, and were secondly proliferated and differentiated via the salvage pathway. S-phase cells associated with a myeloid hematopoiesis increased earlier than those associated with an erythroid hematopoiesis in the recovery phase of in rat bone marrow.
为了确定造血细胞中嘧啶核苷酸合成的调控机制，我们在大鼠经环磷酰胺处理诱导造血功能低下后骨髓造血细胞的恢复阶段，通过流式细胞术DNA分析和溴脱氧尿苷免疫组织化学研究了胸苷酸合成酶和胸苷激酶的活性以及细胞周期。造血细胞首先通过嘧啶核苷酸合成的从头途径进入细胞周期，其次通过补救途径进行增殖和分化。在大鼠骨髓恢复阶段，与髓系造血相关的S期细胞比与红系造血相关的S期细胞更早增加。
