Lanzafame S, Magro G, Colombatti A
Institute of Pathological Anatomy, University of Catania, Italy.
Acta Histochem. 1994 Jun;96(2):219-23. doi: 10.1016/S0065-1281(11)80182-0.
We investigated the distribution of type VI collagen in 36 cases of routinely fixed and paraffin-embedded gynecomastia using an immunoperoxidase method for light microscopic visualization. Four samples of normal male mammary gland tissue were also included as controls. A protease predigestion was essential for the visualization of this extracellular matrix (ECM) glycoprotein. In normal male breast, no immunoreaction for type VI collagen was detected in the stroma surrounding the ducts. Gynecomastia was classified into three histological types: florid (type I), fibrous (type II), and intermediate (type III). Type VI collagen was differentially expressed in the periductal stroma of all types. This collagen was markedly expressed at the early disease stage (type I) when the periductal stroma is highly cellular and vascular. Its expression decreased when periductal stroma undergoing fibrotic transformation (type III) and completely disappeared from the dense periductal stroma of fibrous stage (type II). These findings suggest that type VI collagen is involved in the ECM remodelling occurring in gynecomastia.
我们采用免疫过氧化物酶法进行光镜观察，研究了36例常规固定及石蜡包埋的男性乳腺增生症组织中VI型胶原的分布情况。另外选取4例正常男性乳腺组织样本作为对照。蛋白酶预消化对于这种细胞外基质（ECM）糖蛋白的可视化至关重要。在正常男性乳腺中，导管周围的基质未检测到VI型胶原的免疫反应。男性乳腺增生症分为三种组织学类型： florid型（I型）、纤维型（II型）和中间型（III型）。VI型胶原在所有类型的导管周围基质中均有差异表达。在疾病早期（I型），当导管周围基质细胞丰富且血管化时，这种胶原表达明显；当导管周围基质发生纤维化转变（III型）时，其表达下降，并在纤维期（II型）致密的导管周围基质中完全消失。这些发现表明，VI型胶原参与了男性乳腺增生症中发生的细胞外基质重塑过程。
