Livne E
Division of Morphological Sciences, Bruce Rappapport Faculty of Medicine, Technion-Israel Institute of Technology, Haifa, Israel.
Acta Anat (Basel). 1994;149(3):185-94. doi: 10.1159/000147575.
The ability of human transforming growth factor-beta 1 (hTGF-beta 1) to induce proliferation and matrix synthesis in articular cartilage of aging mice was studied using an organ culture system of mandibular condylar cartilage. An increased incorporation of 3H-thymidine into DNA and of 35S-SO4 into sulfated glycosaminoglycans was observed in cultures supplemented with 1-10 ng/ml TGF-beta and with 1% fetal calf serum. In these cultures numerous clusters of chondrocytes containing a well-developed rough endoplasmic reticulum were seen. Articular cartilage from mature animals often develops osteoarthritic degenerative lesions, thus the induction of cell proliferation and of matrix synthesis may be an indication of a tissue repair process in cartilage from maturing animals.
利用下颌髁突软骨器官培养系统，研究了人转化生长因子β1（hTGF-β1）诱导衰老小鼠关节软骨增殖和基质合成的能力。在添加1-10 ng/ml TGF-β和1%胎牛血清的培养物中，观察到3H-胸腺嘧啶核苷掺入DNA以及35S-硫酸根掺入硫酸化糖胺聚糖的量增加。在这些培养物中可见大量含有发育良好的粗面内质网的软骨细胞簇。成熟动物的关节软骨常出现骨关节炎性退行性病变，因此细胞增殖和基质合成的诱导可能是成熟动物软骨组织修复过程的一个指标。
