Atoji Y, Nakaoka T, Yamamoto Y, Suzuki Y
Laboratory of Veterinary Anatomy, Faculty of Agriculture, Gifu University, Japan.
Acta Anat (Basel). 1994;149(3):203-8. doi: 10.1159/000147577.
Injections of chick erythrocytes were employed to examine the relationship between expression of S-100 protein and antigen stimulation in the giant macrophages (GM) and follicular dendritic cells (FDC) of guinea pig lymph nodes by an immunocytochemical method. Popliteal lymph nodes were observed 1, 3, 6 and 12 h, then 1, 2 and 3 days, and finally 1, 2 and 4 weeks after injection of chick erythrocytes into hind foot pads. All GM in control lymph nodes were dendritic in shape at the cortical surface and strongly immunoreactive to S-100 protein. In lymph nodes of injected animals, three types of GM were observed at the cortical surface: cells were S-100-protein-positive/phagocytosis-positive, S-100-protein-positive/phagocytosis-negative and S-100-protein-negative/phagocytosis-positive. GM that were S-100-protein-negative/phagocytosis-positive appeared from 1 h onwards, reaching a maximum number at 6 h and then decreasing in number with time at the cortical surface. In the medulla, such cells were observed after 1 h, increasing to a maximal level at 6 h and, thereafter, gradually decreasing in number. These results indicate that expression of S-100 protein in GM is altered by antigen stimulation and that GM move in the lymph nodes. The same pattern of expression of S-100 protein was demonstrated in the FDC 1 week after injection.
通过免疫细胞化学方法，采用注射鸡红细胞来检测豚鼠淋巴结的巨噬细胞（GM）和滤泡树突状细胞（FDC）中S-100蛋白表达与抗原刺激之间的关系。将鸡红细胞注射到后足垫后，分别在1、3、6和12小时，然后在1、2和3天，最后在1、2和4周观察腘窝淋巴结。对照淋巴结中的所有GM在皮质表面呈树突状，对S-100蛋白呈强免疫反应性。在注射动物的淋巴结中，在皮质表面观察到三种类型的GM：S-100蛋白阳性/吞噬阳性细胞、S-100蛋白阳性/吞噬阴性细胞和S-100蛋白阴性/吞噬阳性细胞。S-100蛋白阴性/吞噬阳性的GM从1小时起出现，在6小时达到最大数量，然后在皮质表面随时间减少。在髓质中，此类细胞在1小时后观察到，在6小时增加到最大水平，此后数量逐渐减少。这些结果表明，GM中S-100蛋白的表达受抗原刺激影响而改变，且GM在淋巴结中移动。注射1周后，FDC中S-100蛋白的表达呈现相同模式。
