Miyata H, Abe M, Takehana K, Yamaguchi M, Masty J, Iwasa K, Hiraga T
Department of Veterinary Anatomy, Rakuno Gakuen University, Hokkaido, Japan.
Acta Anat (Basel). 1994;149(3):209-14. doi: 10.1159/000147578.
The morphology of reticular cells of the sheathed arteries, in the red pulp of pig spleen, was studied by using transmission electron microscopy; and their histochemical reactivity with periodic acid-thiocarbohydrazide-silver proteinate (PA-TCH-SP). The phagocytic ability was evaluated by injecting colloidal carbon into the splenic artery. Reticular cells of the sheathed arteries were classified as type I and type II cells. Type I cells have a nucleus with scanty chromatin, and the cytoplasm reacts positively to PA-TCH-SP. The PA-TCH-SP-positive granules are considered to be subunits of beta-glycogen particles based on their morphological features. Type II cells have a nucleus with abundant chromatin and are not stained by PA-TCH-SP. Both types of reticular cells are connected with reticular fibers. Results of the colloidal carbon injection showed that type I reticular cells did not ingest carbon particles during the time frame of the experiment, whereas type II reticular cells are phagocytic and ingested carbon.
运用透射电子显微镜对猪脾脏红髓中被膜动脉的网状细胞形态进行了研究；并观察了它们与高碘酸-硫代碳酰肼-银蛋白（PA-TCH-SP）的组织化学反应性。通过向脾动脉注射胶体碳来评估吞噬能力。被膜动脉的网状细胞分为I型和II型细胞。I型细胞的细胞核染色质稀少，细胞质对PA-TCH-SP呈阳性反应。基于其形态特征，PA-TCH-SP阳性颗粒被认为是β-糖原颗粒的亚单位。II型细胞的细胞核染色质丰富，且不被PA-TCH-SP染色。两种类型的网状细胞均与网状纤维相连。胶体碳注射结果显示，在实验时间段内I型网状细胞不摄取碳颗粒，而II型网状细胞具有吞噬作用并摄取了碳。
