Ueda H, Fujimori O
Department of Anatomy, Nagoya City University Medical School, Japan.
Okajimas Folia Anat Jpn. 1995 Aug;72(2-3):109-17. doi: 10.2535/ofaj1936.72.2-3_109.
In the endothelial cells lining the rat splenic blood vessels, neutral carbohydrates were studied by means of combined periodic acid-thiocarbohydrazide-silver protein (PA-TCH-SP) and alpha-amylase digestion methods. In the endothelial cells lining the central and follicular arteries of the spleen, the neutral glycoconjugate-containing surface coat of the luminal plasma membrane and related pinocytotic invaginations and vesicles in the apical cytoplasm were strikingly distinguished, as compared with those in the cells lining the splenic sinuses. In contrast, cytoplasmic glycogen particles in the sinus endothelial cells were apparently larger in amount than those in the arteriolar endothelial cells. Such cytochemical variations of neutral carbohydrates with the arteriolar and venous vessels of the rat spleen were discussed with special reference to varying cytophysiological functions of the endothelial cells with the different segments of the splenic blood vessels.
采用高碘酸 - 硫代碳酰肼 - 银蛋白（PA - TCH - SP）联合法及α - 淀粉酶消化法，对大鼠脾脏血管内皮细胞中的中性碳水化合物进行了研究。与脾窦内皮细胞相比，脾中央动脉和滤泡动脉内皮细胞中，管腔质膜含中性糖缀合物的表面被膜以及顶端胞质中相关的胞饮内陷和小泡非常明显。相反，窦内皮细胞中的胞质糖原颗粒数量明显多于小动脉内皮细胞。结合大鼠脾脏小动脉和静脉血管内皮细胞不同的细胞生理功能，对中性碳水化合物的这种细胞化学变化进行了讨论。
